Characteristics of graft-versus-host disease after Lewis-to-brown-Norway rat small bowel transplantation.

Flow Cytometric Analvsis. The spleens were taken from the recipients of SBTx or BMTx 2 to 6 days after the onset of GVHD for two-color flow cytometric analysis. Splenocytes were stained with McAb 163. which is specific for the RTIAI antigens on LEW. or with McAb 163. which is specificforthe RTIA" antigen on BN (gift from H.W. Kunz. Department of Pathology. University of Pittsburgh. Pittsburgh. Pal in conjunction with McAbs to lymphocyte lineage markers (Serotec. Oxford. Englandl. including W312S tmt CD4 and macrophages). OX8 (rat CDS and some NK cells). R7.3 (a(3TCR. rat T cells). OX33 (rat B cellsl and 3.:!.3. (mt NK cells. gift of W. Chambers. Pittsburgh Cancer Institute. Pittsburgh. Pal. Direc" Immllm~lI()r('scence. Recipient kidneys were removed :! to 6 days after the onset of GVHD. Cryostat sections were stained with FITC-conjugated goat anllrallgG (Sera Labordtory. Westbury. NY) or IgM (Jackson. West Grove. Pal to detect rat immunoglobulin binding to the renal glomenllus.